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1 October 2001 Developmental Regulation of Placental Insulin-Like Growth Factor (IGF)-II and IGF-Binding Protein-1 and -2 Messenger RNA Expression During Primate Pregnancy
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Abstract

The present study was conducted to determine the developmental expression of placental insulin-like growth factor (IGF)-II, IGF-binding protein (IGFBP)-1 and -2, and IGF-II receptor mRNA expression during baboon pregnancy and whether estrogen, the levels of which increase with advancing pregnancy, regulates placental trophoblast IGF-II mRNA expression. Levels of the IGF-II 6.1-kilobase (kb) and 4.9-kb mRNA transcripts determined by Northern blot analysis progressively increased three- to fourfold in placental syncytiotrophoblast and whole-villous tissue between early (Day 60), mid (Day 100), and late (Day 170) baboon gestation (term = 184 days). In contrast, syncytiotrophoblast IGFBP-1 and -2 mRNA levels decreased, and IGF-II receptor mRNA expression remained relatively constant, with advancing baboon pregnancy. Placental cytotrophoblast IGF-II mRNA levels determined by competitive reverse transcription-polymerase chain reaction on Day 54 of gestation were increased (P < 0.05) almost twofold at 18 h after acute administration of estradiol to baboons, whereas long-term estrogen treatment had no effect. We propose that these changes in trophoblast IGF expression would provide a mechanism for enhancing net bioavailability and bioreactivity of IGF-II locally to promote the growth and development of the placenta and, consequently, of the fetus during primate pregnancy.

William G. Zollers, Jeffery S. Babischkin, Gerald J. Pepe, and Eugene D. Albrecht "Developmental Regulation of Placental Insulin-Like Growth Factor (IGF)-II and IGF-Binding Protein-1 and -2 Messenger RNA Expression During Primate Pregnancy," Biology of Reproduction 65(4), 1208-1214, (1 October 2001). https://doi.org/10.1095/biolreprod65.4.1208
Published: 1 October 2001
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