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1 March 2002 Expression Patterns of Retinoid X Receptors, Retinaldehyde Dehydrogenase, and Peroxisome Proliferator Activated Receptor Gamma in Bovine Preattachment Embryos
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In cattle, administration of retinol at the time of superovulation has been indirectly associated with enhanced developmental potential of the embryo. Vitamin A and its metabolites influence several developmental processes by interacting with 2 different types of nuclear receptors, retinoic acid receptors and retinoid X receptors (RXRs). Given the limited information available concerning the RXR-mediated retinoid signaling system, particularly in species other than rodents, this study was performed to gain insight into the potential role of retinoid signaling during preattachment embryo development in the cow. Bovine embryos were produced in vitro from oocytes harvested from abattoir ovaries and frozen in liquid nitrogen at the oocyte, 2-, 4-, 8-, and 16- to 20-cell, morula, blastocyst, and hatched blastocyst stages. Reverse transcription polymerase chain reaction (PCR) and whole mount in situ hybridization were utilized to investigate mRNA expression for RXRα, RXRβ, RXRγ, alcohol dehydrogenase I (ADH-I), retinaldehyde dehydrogenase 2 (RALDH2), peroxisome proliferator activated receptor gamma (PPARγ), and glyceraldehyde-3-phosphate dehydrogenase. Transcripts for RXRα, RXRβ, RALDH2, and PPARγ were detected in all stages beginning from the oocyte through to the hatched blastocyst. Whole mount in situ hybridization performed using digoxigenin-labeled antisense probes detected all 4 transcripts in both the inner cell mass and the trophectoderm of hatched blastocysts. PCR products obtained for ADH-I exhibited very low homology to known human and mouse sequences. Immunohistochemistry was performed using polyclonal anti-rabbit antibodies against RXRβ and PPARγ to investigate whether these embryonic mRNAs were translated to the mature protein. Strong immunostaining was observed for both RXRβ and PPARγ in the trophectoderm and inner cell mass cells of intact and hatched blastocysts. Messenger RNA was not detected at any stage for RXRγ. Expression of mRNA for RXRα, RXRβ, RALDH2, and PPARγ suggests that the early embryo may be competent to synthesize retinoic acid and regulate gene expression during preattachment development in vitro.

M. Mohan, J. R. Malayer, R. D. Geisert, and G. L. Morgan "Expression Patterns of Retinoid X Receptors, Retinaldehyde Dehydrogenase, and Peroxisome Proliferator Activated Receptor Gamma in Bovine Preattachment Embryos," Biology of Reproduction 66(3), 692-700, (1 March 2002).
Received: 2 August 2001; Accepted: 1 October 2001; Published: 1 March 2002

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