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1 June 2002 Expression of Aquaporin 9 in the Adult Rat Epididymal Epithelium Is Modulated by Androgens
Nuria Pastor-Soler, Corinne Isnard-Bagnis, Carol Herak-Kramberger, Ivan Sabolic, Alfred Van Hoek, Dennis Brown, Sylvie Breton
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Abstract

Reabsorption of fluid and solutes across the epithelium lining the male excurrent duct is important for adequate sperm maturation, concentration, and storage. Water channels contribute to water movement across epithelia in many tissues. Aquaporin 9 (AQP9) is abundantly expressed in the apical membrane of principal cells that line the epididymis, and in reabsorptive and secretory epithelial cells of the male reproductive tract. In this study we show that the nonsteroidal antiandrogen flutamide, given to adult rats at a dose of 50 mg kg−1 day−1 for 2 wk via osmotic minipumps significantly decreased the amount of AQP9 in the epididymis. This down-regulation was observed by immunofluorescence of cryostat tissue sections and by Western blotting of epididymal brush border membrane preparations. In addition, castrated adult rats showed lower levels of epididymal AQP9 compared with adult controls, whereas systemic testosterone treatment of castrated adult rats induced a recovery of the expression of AQP9 to control levels. These data indicate that the expression of AQP9, a likely candidate for apical transepithelial fluid and solute transport in several regions of the male reproductive tract, is modulated by androgens in the adult rat epididymis.

Nuria Pastor-Soler, Corinne Isnard-Bagnis, Carol Herak-Kramberger, Ivan Sabolic, Alfred Van Hoek, Dennis Brown, and Sylvie Breton "Expression of Aquaporin 9 in the Adult Rat Epididymal Epithelium Is Modulated by Androgens," Biology of Reproduction 66(6), 1716-1722, (1 June 2002). https://doi.org/10.1095/biolreprod66.6.1716
Received: 30 October 2001; Accepted: 1 January 2002; Published: 1 June 2002
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