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1 July 2002 Molecular Cloning and Characterization of oppo 1: A Haploid Germ Cell-Specific Complementary DNA Encoding Sperm Tail Protein
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Abstract

We isolated a cDNA clone specifically expressed during spermatogenesis from a subtracted cDNA library of mouse testis. The cDNA consisted of 1085 nucleotides and had an open reading frame of 870 nucleotides encoding a putative protein of 290 amino acid residues. Northern blot analysis revealed a 1.2-kilobase mRNA exclusively expressed in the testis in adult mice; the mRNA was first detected late pachytene stage, and expression increased as the animals matured. The protein encoded by the mRNA had a molecular weight of ∼33 kDa by Western blot analysis, and was localized to occupy the flagella from the connecting piece through the principal piece. We named this newly isolated gene oppo 1, and we suggest that it plays an important role in sperm tail structure and/or sperm movement.

Yoshihiro Nakamura, Hiromitsu Tanaka, Minoru Koga, Yasushi Miyagawa, Naoko Iguchi, Carlos Egydio de Carvalho, Kentaro Yomogida, Masami Nozaki, Hiroshi Nojima, Kiyomi Matsumiya, Akihiko Okuyama, and Yoshitake Nishimune "Molecular Cloning and Characterization of oppo 1: A Haploid Germ Cell-Specific Complementary DNA Encoding Sperm Tail Protein," Biology of Reproduction 67(1), 1-7, (1 July 2002). https://doi.org/10.1095/biolreprod67.1.1
Received: 27 November 2001; Accepted: 1 January 2002; Published: 1 July 2002
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