Translator Disclaimer
1 July 2002 Nuclei of Nonviable Ovine Somatic Cells Develop into Lambs after Nuclear Transplantation
Author Affiliations +
Abstract

Here we report on the successful reprogramming of nuclei from somatic cells rendered nonviable by heat treatment. Granulosa cells from adult sheep were heated to nonphysiological temperatures (55°C or 75°C) before their nuclei were injected into enucleated metaphase II oocytes. Reprogramming was demonstrated by the capacity of the reconstructed embryos to develop to the blastocyst stage in vitro and into fetuses and viable offspring in suitable foster mothers. To our knowledge, this is the first report of cloned mammalian offspring originating from nonviable cells. In addition, our experiments show that heat-treating donor nuclei destabilizes higher-order features of chromatin (but leaves intact its nucleosomal organization) and results in a high proportion of reconstructed embryos developing to the blastocyst stage and beyond.

Pasqualino Loi, Michael Clinton, Barbara Barboni, Josef Fulka, Jr., Pietro Cappai, Robert Feil, Robert M. Moor, and Grazyna Ptak "Nuclei of Nonviable Ovine Somatic Cells Develop into Lambs after Nuclear Transplantation," Biology of Reproduction 67(1), 126-132, (1 July 2002). https://doi.org/10.1095/biolreprod67.1.126
Received: 4 October 2001; Accepted: 1 January 2002; Published: 1 July 2002
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top