Translator Disclaimer
1 August 2002 Steroidogenic Acute Regulatory Protein Expression Is Decreased in the Adrenal Gland of the Growth-Restricted Sheep Fetus During Late Gestation
Author Affiliations +
Abstract

Functional development of the adrenal cortex is critical for fetal maturation and postnatal survival. In the present study, we have determined the developmental profile of expression of the mRNA and protein of an essential cholesterol-transporting protein, steroidogenic acute regulatory protein (StAR), in the adrenal of the sheep fetus. We have also investigated the effect of placental restriction (PR) on the expression of StAR mRNA and protein in the growth-restricted fetus. Adrenal glands were collected from fetal sheep at 82–91 days (n = 10), 125–133 days (n = 10), and 140–144 days (n = 9) and from PR fetuses at 141–145 days gestation (n = 9) (term = 147 ± 3 days gestation). The adrenal StAR mRNA:18S rRNA increased (P < 0.05) between 125 days (7.44 ± 1.61) and 141–144 days gestation (13.76 ± 1.88). There was also a 13-fold increase (P < 0.05) in the amount of adrenal StAR protein between 133 and 144 days gestation in these fetuses. However, the amount of StAR protein (6.9 ± 1.7 arbitrary densitometric units [AU]/μg adrenal protein) in the adrenal of the growth-restricted fetal sheep was significantly reduced, when compared with the expression of StAR protein (17.1 ± 1.9 AU/μg adrenal protein) in adrenals from the age-matched control group. In summary, there is a developmental increase in the expression of StAR mRNA and protein in the fetal sheep adrenal during the prepartum period when adrenal growth and steroidogenesis is dependent on ACTH stimulation. We have found that, while the level of expression of StAR protein is decreased in the adrenal gland of the growth-restricted fetus during late gestation, this does not impair adrenal steroidogenesis. Our data also suggest that the stimulation of adrenal growth and steroidogenesis in the growth-restricted fetus may not be ACTH dependent.

Catherine L. Coulter, I. Caroline McMillen, Ian M. Bird, and Mark D. Salkeld "Steroidogenic Acute Regulatory Protein Expression Is Decreased in the Adrenal Gland of the Growth-Restricted Sheep Fetus During Late Gestation," Biology of Reproduction 67(2), 584-590, (1 August 2002). https://doi.org/10.1095/biolreprod67.2.584
Received: 15 January 2002; Accepted: 1 March 2002; Published: 1 August 2002
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top