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1 December 2002 Molecular Characterization of Genomic Activities at the Onset of Zygotic Transcription in Mammals
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Abstract

In rabbit embryos, zygotic transcripts are required for the development of the embryo only from the 8- to 16-cell stage onward, more than 44 h after fertilization (i.e., zygotic gene activation; ZGA). In order to characterize the first zygotic transcripts expressed in this species we used a suppression subtractive hybridization approach to isolate RNA that was present after the major transcriptional activation (morula stage), but absent at the 1-cell stage as maternal transcripts. One hundred fourteen differentially expressed inserts were selected and sequenced. A statistical analysis of expression patterns throughout the preimplantation period of development shows that genes transcribed from ZGA onward follow different patterns of expression. Considering their early post-ZGA behavior, we describe at least two main patterns: a gradual increase from ZGA onward, and a sharp increase in expression at ZGA followed by a marked decrease at the morula stage. Our data show that both ZGA and some early post-ZGA events are involved in the establishment of specific patterns of embryonic gene expression.

Sylviane Pacheco-Trigon, Christelle Hennequet-Antier, Jean-François Oudin, François Piumi, Jean-Paul Renard, and Véronique Duranthon "Molecular Characterization of Genomic Activities at the Onset of Zygotic Transcription in Mammals," Biology of Reproduction 67(6), 1907-1918, (1 December 2002). https://doi.org/10.1095/biolreprod67.6.1907
Received: 24 August 2001; Accepted: 1 July 2002; Published: 1 December 2002
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