Translator Disclaimer
1 July 2003 γ-Tubulin Overexpression in Sertoli Cells In Vivo. II: Retention of Spermatids, Residual Bodies, and Germ Cell Apoptosis
Author Affiliations +
Abstract

The degree of germ cell dependence on Sertoli cell-mediated activities has been a subject of considerable attention. Sertoli cell secretory pathways have been extensively studied both in an effort to understand their normal physiologic roles and as targets for pharmacologic and toxicant activity. To determine the degree to which normal spermatogenesis depends on key functions of the Sertoli cell microtubule network, adenoviral vectors that overexpress the microtubule nucleating protein, γ-tubulin, were delivered to Sertoli cells in vivo. γ-Tubulin overexpression disrupts the Sertoli cell microtubule network (as described in the companion article); leads to gross disorganization of the seminiferous epithelium, inducing retention of spermatids and residual bodies; and causes germ cell apoptosis. These data are consistent with earlier studies in which toxicants and pharmacologic agents were used to disrupt microtubule networks. These data confirm that Sertoli cell microtubule networks play an important role in maintaining the organization of the seminiferous epithelium and that in the absence of an intact Sertoli cell microtubule network, germ cell viability is impaired.

Shawna L. Fleming, Peter R. Shank, and Kim Boekelheide "γ-Tubulin Overexpression in Sertoli Cells In Vivo. II: Retention of Spermatids, Residual Bodies, and Germ Cell Apoptosis," Biology of Reproduction 69(1), 322-330, (1 July 2003). https://doi.org/10.1095/biolreprod.102.011817
Received: 26 September 2002; Accepted: 1 March 2003; Published: 1 July 2003
JOURNAL ARTICLE
9 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top