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1 August 2003 Dynamic Regulation of Expression of Colony-Stimulating Factor 1 in the Reproductive Tract of Cattle During the Estrous Cycle and in Pregnancy
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Abstract

Colony-stimulating factor 1 (CSF-1) is a hematopoetic cytokine that also plays an important role in placental physiology. We report here the molecular cloning of two alternative splice variants of the bovine gene coding for a putative secreted and a membrane-bound form of the cytokine and the dynamic regulation of expression in the reproductive tract of cattle during the estrous cycle and pregnancy. Bovine CSF-1 was expressed mainly as the 3- and 4-kilobase (kb) transcripts, but 1.4- and 0.8-kb mRNAs were also detected in Day 50–70 pregnant uterine tissue. During the estrous cycle, both the 4- and 3-kb mRNAs were present, but the 3-kb putative membrane-bound form was more abundant than the 4-kb secreted form during diestrus. This pattern of expression was reversed in pregnancy, so that the exponential increase in CSF-1 expression seen during pregnancy was due predominantly to increased abundance of the 4-kb transcript. The change in the 4-kb:3-kb ratio was detected between Day 14 and Day 17, approximately the time of maternal recognition of pregnancy. Thus, CSF-1 was identified as one gene whose expression in the uterus might be altered early in response to the presence of the conceptus. CSF-1 was also expressed in the extraembryonic membranes of the conceptus and in the trophoblastic cells of the fetal cotyledons after the formation of the placentomes. The high level of CSF-1 expression during bovine pregnancy in uteroplacental tissues is consistent with its proposed role in placental physiology.

Rita S. F. Lee, Ning Li, Anita M. Ledgard, and Jeffrey W. Pollard "Dynamic Regulation of Expression of Colony-Stimulating Factor 1 in the Reproductive Tract of Cattle During the Estrous Cycle and in Pregnancy," Biology of Reproduction 69(2), 518-528, (1 August 2003). https://doi.org/10.1095/biolreprod.102.013748
Received: 27 November 2002; Accepted: 1 March 2003; Published: 1 August 2003
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