Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1

Mito Kanatsu-Shinohara; Narumi Ogonuki; Kimiko Inoue; Hiromi Miki; Atsuo Ogura; Shinya Toyokuni; Takashi Shinohara

doi:10.1095/biolreprod.103.017012

Translator Disclaimer

You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.

Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.

1 August 2003 Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells

Mito Kanatsu-Shinohara, Narumi Ogonuki, Kimiko Inoue, Hiromi Miki, Atsuo Ogura, Shinya Toyokuni, Takashi Shinohara

Author Affiliations +

Mito Kanatsu-Shinohara,^1,* Narumi Ogonuki,² Kimiko Inoue,³ Hiromi Miki,³ Atsuo Ogura,³ Shinya Toyokuni,² Takashi Shinohara¹

¹aHorizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-
²bDepartment of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyo
³cThe Institute of Physical and Chemical Research (RIKEN), Bioresource Center, Ibaraki 305-0074, Japa

^*Correspondence: Mito Kanatsu-Shinohara, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Yoshida-Konoe, Sakyo-ku, Kyoto 606-8501, Japan. FAX: 81 75 753 9281; mitos@mfour.med.kyoto-u.ac.jp

Biology of Reproduction, 69(2):612-616 (2003). https://doi.org/10.1095/biolreprod.103.017012

ARTICLE
FIGURES & TABLES
REFERENCES
CITED BY
DOWNLOAD PAPER SAVE TO MY LIBRARY

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

Price: ADD TO CART

Includes PDF & HTML, when available

This article is only available to subscribers.
It is not available for individual sale.

RIGHTS & PERMISSIONS

Get copyright permission

This will count as one of your downloads.

You will have access to both the presentation and article (if available).

DOWNLOAD NOW

This content is available for download via your institution's subscription. To access this item, please sign in to your personal account.

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account

My Library

You currently do not have any folders to save your paper to! Create a new folder below.

Abstract

Spermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>10¹⁴-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.

Citation Download Citation

Mito Kanatsu-Shinohara, Narumi Ogonuki, Kimiko Inoue, Hiromi Miki, Atsuo Ogura, Shinya Toyokuni, and Takashi Shinohara "Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells," Biology of Reproduction 69(2), 612-616, (1 August 2003). https://doi.org/10.1095/biolreprod.103.017012

Received: 9 March 2003; Accepted: 1 April 2003; Published: 1 August 2003

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Password Forgot your password?

Show

Remember Email on this computer

Remember Password

No BioOne Complete account? Create an account
or Access Institutional Sign In via Shibboleth or OpenAthens

PURCHASE THIS CONTENT

PURCHASE SINGLE ARTICLE

This article is only available to subscribers.
It is not available for individual sale.

RIGHTS & PERMISSIONS