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1 September 2004 Expression and Functional Significance of Mouse Paraspeckle Protein 1 on Spermatogenesis
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Abstract

Paraspeckle protein 1 (PSP1) in humans is a recently identified component protein of a novel nuclear body, paraspeckle. The protein has a DBHS (Drosophila behavior, human splicing) motif that is found in PSF and p54nrb/NonO proteins. These DBHS-containing proteins have been reported to be involved in various nuclear events such as DNA replication, transcription, and mRNA processing. Here we show that mouse paraspeckle protein 1 (mPSP1; encoded by the Pspc1 gene) has two isoforms with different C-termini lengths. Abundant expression of the longer isoform (mPSP1-α) and the shorter one (mPSP1-β) were observed in testis and kidney, respectively. Transiently expressed mPSP1-α was localized in nuclei, but mPSP1-β was localized in both nuclei and cytoplasm. These observations suggest that alternative splicing regulates tissue distribution and subcellular localization. Like other DBHS-containing proteins, mPSP1 has RNA-binding activity. In mouse testis, mPSP1-α was found in the nuclear matrix fraction. Furthermore, by coimmunoprecipitation, we confirmed that mPSP1 interacts with other DBHS-containing proteins, PSF and p54nrb/NonO. Therefore, we conclude that mPSP1 may regulate multiple phases of important nuclear events during spermatogenesis.

Reiko Myojin, Sho Kuwahara, Tomohiko Yasaki, Takahito Matsunaga, Takayuki Sakurai, Minoru Kimura, Seiichi Uesugi, and Yasuyuki Kurihara "Expression and Functional Significance of Mouse Paraspeckle Protein 1 on Spermatogenesis," Biology of Reproduction 71(3), 926-932, (1 September 2004). https://doi.org/10.1095/biolreprod.104.028159
Received: 4 February 2004; Accepted: 1 April 2004; Published: 1 September 2004
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