Translator Disclaimer
1 November 2004 BARD1 Expression During Spermatogenesis Is Associated with Apoptosis and Hormonally Regulated
Author Affiliations +

The BRCA1-binding RING-finger domain protein BARD1 may act conjointly with BRCA1 in DNA repair and in ubiquitination, but it may also induce apoptosis in a BRCA1-independent manner. In this study, we have investigated BARD1 expression during spermatogenesis. In contrast with BRCA1, which is expressed only in meiotic spermatocytes and early round spermatids, BARD1 is expressed during all stages of spermatogenesis. However, while spermatogonia expressed full-length BARD1 mRNA, later stages of spermatocyte precursors express predominantly a novel, shorter splice form BARD1β. BARD1β lacks the BRCA1-interacting RING finger but maintains its proapoptotic activity. Consistently, BRCA1 can counteract the proapoptotic activity of full-length BARD1 but not of BARD1β. Several lines of evidence suggest that BARD1 is involved in proapoptotic signaling in testis: i) both BARD1 isoforms are mostly found in cells that stain positive for TUNEL, Bax, and activated caspase 3; ii) BARD1β, capable of inducing apoptosis even in the presence of BRCA1, is specifically expressed in BRCA1-positive later stages of spermatogenesis; iii) antiapoptotic hormonal stimulation leads to BARD1 downregulation; and iv) BARD1 expression is associated with human pathologies causing sterility due to increased germ cell death. Our data suggest that full-length BARD1 might be involved in apoptotic control in spermatogonia and primary spermatocytes, while a switch to the BRCA1-independent BARD1β might be necessary to induce apoptosis in BRCA1-expressing meiotic spermatocytes and early round spermatids.

Anis Feki, Charles-Edwards Jefford, Philippe Durand, Jean Harb, Hervé Lucas, Karl-Heinz Krause, and Irmgard Irminger-Finger "BARD1 Expression During Spermatogenesis Is Associated with Apoptosis and Hormonally Regulated," Biology of Reproduction 71(5), 1614-1624, (1 November 2004).
Received: 15 March 2004; Accepted: 1 June 2004; Published: 1 November 2004

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top