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1 November 2004 Differential Expression and Antibacterial Activity of Epididymis Protein 2 Isoforms in the Male Reproductive Tract of Human and Rhesus Monkey (Macaca mulatta)
Maria Christina W. Avellar, Luciana Honda, Katherine G. Hamil, Suresh Yenugu, Gail Grossman, Peter Petrusz, Frank S. French, Susan H. Hall
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Abstract

The epididymis protein 2 (EP2) gene, the fusion of two ancestral β-defensin genes, is highly expressed in the epididymis and subject to species-specific regulation at the levels of promoter selection, transcription, and mRNA splicing. EP2 mRNA expression is also androgen dependent, and at least two of the secreted proteins bind spermatozoa. Alternative splicing produces more than 17 different EP2 mRNA variants. In this article, the expression of EP2 variants was profiled in different tissues from the human and rhesus monkey (Macaca mulatta) male reproductive tract using reverse transcriptase-polymerase chain reaction. Different EP2 mRNA variants were identified not only in human and rhesus testis and epididymis but also in the novel sites, seminal vesicle and prostate. Immunolocalization of EP2 protein in epithelial cells from rhesus and human seminal vesicle demonstrated that EP2 transcripts are translated in these tissues. In addition, two novel splicing variants, named EP2R and EP2S, were discovered. EP2C was the only splice variant expressed in all tissues tested from rhesus monkey. However, expression was not detected in human testis or seminal vesicle. For the first time, bactericidal function was demonstrated for EP2C, EP2K, and EP2L. Taken together, the results indicate that EP2 expression is more widespread in the male reproductive tract than realized previously. Whereas the activity of every EP2 variant tested thus far is antibacterial, further investigation may reveal additional physiological roles for EP2 peptides in the primate male reproductive tract.

Maria Christina W. Avellar, Luciana Honda, Katherine G. Hamil, Suresh Yenugu, Gail Grossman, Peter Petrusz, Frank S. French, and Susan H. Hall "Differential Expression and Antibacterial Activity of Epididymis Protein 2 Isoforms in the Male Reproductive Tract of Human and Rhesus Monkey (Macaca mulatta)," Biology of Reproduction 71(5), 1453-1460, (1 November 2004). https://doi.org/10.1095/biolreprod.104.031740
Received: 6 May 2004; Accepted: 1 June 2004; Published: 1 November 2004
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