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1 March 2005 Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development
Martha Pfister-Genskow, Cena Myers, Lynette A. Childs, Jenine C. Lacson, Thomas Patterson, Jeffery M. Betthauser, Paul J. Goueleke, Richard W. Koppang, Gail Lange, Patricia Fisher, Steven R. Watt, Erik J. Forsberg, Ying Zheng, Gregory H. Leno, Richard M. Schultz, Bing Liu, Chiranjeet Chetia, Xiao Yang, Ina Hoeschele, Kenneth J. Eilertsen
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Abstract

Using an interwoven-loop experimental design in conjunction with highly conservative linear mixed model methodology using estimated variance components, 18 genes differentially expressed between nuclear transfer (NT)- and in vitro fertilization (IVF)-produced embryos were identified. The set is comprised of three intermediate-filament protein genes (cytokeratin 8, cytokeratin 19, and vimentin), three metabolic genes (phosphoribosyl pyrophosphate synthetase 1, mitochondrial acetoacetyl-coenzyme A thiolase, and α-glucosidase), two lysosomal-related genes (prosaposin and lysosomal-associated membrane protein 2), and a gene associated with stress responses (heat shock protein 27) along with major histocompatibility complex class I, nidogen 2, a putative transport protein, heterogeneous nuclear ribonuclear protein K, mitochondrial 16S rRNA, and ES1 (a zebrafish orthologue of unknown function). The three remaining genes are novel. To our knowledge, this is the first report comparing individual embryos produced by NT and IVF using cDNA microarray technology for any species, and it uses a rigorous experimental design that emphasizes statistical significance to identify differentially expressed genes between NT and IVF embryos in cattle.

Martha Pfister-Genskow, Cena Myers, Lynette A. Childs, Jenine C. Lacson, Thomas Patterson, Jeffery M. Betthauser, Paul J. Goueleke, Richard W. Koppang, Gail Lange, Patricia Fisher, Steven R. Watt, Erik J. Forsberg, Ying Zheng, Gregory H. Leno, Richard M. Schultz, Bing Liu, Chiranjeet Chetia, Xiao Yang, Ina Hoeschele, and Kenneth J. Eilertsen "Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development," Biology of Reproduction 72(3), 546-555, (1 March 2005). https://doi.org/10.1095/biolreprod.104.031799
Received: 25 May 2004; Accepted: 1 September 2004; Published: 1 March 2005
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