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1 December 2005 Tn5 Transposase-Mediated Mouse Transgenesis
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Abstract

We have developed a novel method for mouse transgenesis. The procedure relies on a hyperactive Tn5 transposase to insert a transgene into mouse chromosomes during intracytoplasmic sperm injection. This procedure integrates foreign DNA into the mouse genome with dramatically increased effectiveness as compared to conventional methods such as pronuclear microinjection and traditional sperm injection-mediated transgenesis. Our data indicate that with this method, transgenic mice, both hybrids and inbreds, can be produced more consistently and with lower numbers of manipulated oocytes required for traditional microinjection methods. The transposase-mediated transgenesis technique is also effective with round spermatids, offering the potential for rescuing the fertility of azoospermic animals using sperm precursor cells.

Ryota Suganuma, Pawel Pelczar, Jean François Spetz, Barbara Hohn, Ryuzo Yanagimachi, and Stefan Moisyadi "Tn5 Transposase-Mediated Mouse Transgenesis," Biology of Reproduction 73(6), 1157-1163, (1 December 2005). https://doi.org/10.1095/biolreprod.105.044669
Received: 15 June 2005; Accepted: 1 July 2005; Published: 1 December 2005
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