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1 March 2006 Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists
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Capacitation of mammalian sperm, including alterations in flagellar motility, is presumably modulated by chemical signals encountered in the female reproductive tract. This work investigates signaling pathways for adenosine and catecholamine agonists that stimulate sperm kinetic activity. We show that 2-chloro-2′-deoxyadenosine and isoproterenol robustly accelerate flagellar beat frequency with EC50s near 10 and 0.05 μM, respectively. The several-fold acceleration is maximal by 60 sec. Although extracellular Ca2 is required for agonist action on the flagellar beat, agonist treatment does not elevate sperm cytosolic [Ca2 ] but does increase cAMP content. Acceleration does not require the conventional transmembrane adenylyl cyclase ADCY3, since it persists in sperm of ADCY3 knockout mice and in wild-type sperm in the presence of the inhibitors of conventional adenylyl cyclases SQ-22536, MDL-12330A, or 2′, 5′-dideoxyadenosine. In contrast, the acceleration by these agents is absent in sperm that lack the predominant atypical adenylyl cyclase, SACY. Responses to these agonists are also absent in sperm from mice lacking the sperm-specific Cα2 catalytic subunit of protein kinase A (PRKACA). Agonist responses also are strongly suppressed in wild-type sperm by the protein kinase inhibitor H-89. These results show that adenosine and catecholamine analogs activate sperm motility by mechanisms that require extracellular Ca2 , the atypical sperm adenylyl cyclase, cAMP, and protein kinase A.

Sonya M. Schuh, Anne E. Carlson, G. Stanley McKnight, Marco Conti, Bertil Hille, and Donner F. Babcock "Signaling Pathways for Modulation of Mouse Sperm Motility by Adenosine and Catecholamine Agonists," Biology of Reproduction 74(3), 492-500, (1 March 2006).
Received: 23 September 2005; Accepted: 1 November 2005; Published: 1 March 2006

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