Translator Disclaimer
1 October 2006 Restoration of Spermatogenesis and Male Fertility by Transplantation of Dispersed Testicular Cells in the Chicken
Author Affiliations +
Abstract

Transplantation of male germ cells into sterilized recipients has been widely used in mammals for conventional breeding and transgenesis purposes. This study presents a workable approach for germ cell transplantation between male chickens. Testicular cells from adult and prepubertal donors were dispersed and transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient seminiferous epithelium up to the production of heterologous sperm in about 50% of transplanted males. In comparison to males transplanted with testicular cell preparations from adult donors, in which the first ejaculates with sperm were recovered about 5 wk after transfer, a substantial interval (about 10 wk) was necessary to obtain ejaculates after the transfer of testicular cells from prepubertal donors. However, in both cases, recipient males produced ejaculates capable of fertilizing ova and producing progeny expressing donor genes.

Pavel Trefil, Alena Micáková, Jitka Mucksová, Jirí Hejnar, Martin Poplstein, Murray R. Bakst, Jirí Kalina, and Jean-Pierre Brillard "Restoration of Spermatogenesis and Male Fertility by Transplantation of Dispersed Testicular Cells in the Chicken," Biology of Reproduction 75(4), 575-581, (1 October 2006). https://doi.org/10.1095/biolreprod.105.050278
Received: 14 December 2005; Accepted: 1 June 2006; Published: 1 October 2006
JOURNAL ARTICLE
7 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top