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1 February 2008 Store-Operated Calcium Entry in Human Oocytes and Sensitivity to Oxidative Stress
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Calcium signaling is a cellular event that plays a key role at many steps of fertilization and early development. However, little is known regarding the contribution of extracellular Ca2 influx into the cell to this signaling in gametes and early embryos. To better know the significance of calcium entry on oocyte physiology, we have evaluated the mechanism of store-operated calcium entry (SOCE) in human metaphase II (MII) oocytes and its sensitivity to oxidative stress, one of the major factors implicated in the outcome of in vitro fertilization (IVF) techniques. We show that depletion of intracellular Ca2 stores through inhibition of sarco(endo)plasmic Ca2 -ATPase with thapsigargin triggers Ca2 entry in resting human oocytes. Ba2 and Mn2 influx was also stimulated following inhibition, and Ca2 entry was sensitive to pharmacological inhibition because the SOCE blocker 2-aminoethoxydiphenylborate (2-APB) reduced calcium and barium entry. These results support the conclusion that there is a plasma membrane mechanism responsible for the capacitative divalent cation entry in human oocytes. Moreover, the Ca2 entry mechanism described in MII oocytes was found to be highly sensitive to oxidative stress. Hydrogen peroxide, at micromolar concentrations that could mimic culture conditions in IVF, elicited an increase of [Ca2 ]i that was dependent on the presence of extracellular Ca2 . This rise was preventable by 2-APB, indicating that it was mainly due to the enhanced influx through store-operated calcium channels. In sum, our results demonstrate the occurrence of SOCE in human MII oocytes and the modification of this pathway due to oxidative stress, with possible consequences in IVF.

Francisco Javier Martín-Romero, Jose Ramón Ortíz-de-Galisteo, Javier Lara-Laranjeira, Jose Antonio Domínguez-Arroyo, Ernesto González-Carrera, and Ignacio S. Álvarez "Store-Operated Calcium Entry in Human Oocytes and Sensitivity to Oxidative Stress," Biology of Reproduction 78(2), 307-315, (1 February 2008).
Received: 30 July 2007; Accepted: 1 November 2007; Published: 1 February 2008

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