Translator Disclaimer
1 July 2008 Extracellular Adenosine 5′-Triphosphate Alters Motility and Improves the Fertilizing Capability of Mouse Sperm
Author Affiliations +

Extracellular adenosine 5′-triphosphate (ATPe) treatment of human sperm has been implicated in improving in vitro fertilization (IVF) results. We used the mouse model to investigate mechanisms of action of ATPe on sperm. ATPe treatment significantly enhanced IVF success as indicated by both rate of pronuclear formation and percentage cleavage to the 2-cell stage. However, ATPe did not increase the percentage of sperm undergoing spontaneous acrosomal exocytosis nor change the pattern of protein tyrosine phosphorylation normally observed in capacitated sperm. ATPe altered sperm motility parameters; in particular, both noncapacitated and capacitated sperm swam faster and straighter. The percentage of hyperactivated sperm did not increase in capacitated ATPe-treated sperm compared to control sperm. ATPe induced a rapid increase in the level of intracellular calcium that was inhibited by two distinct P2 purinergic receptor inhibitors, confirming that these receptors have an ionotropic role in sperm function. The observed motility changes likely explain, in part, the improved fertilizing capability when ATPe-treated sperm were used in IVF procedures and suggest a mechanism by which ATPe treatment may be beneficial for artificial reproductive techniques.

Esmeralda Rodríguez-Miranda, Mariano G. Buffone, Scott E. Edwards, Teri S. Ord, Kathleen Lin, Mary D. Sammel, George L. Gerton, Stuart B. Moss, and Carmen J. Williams "Extracellular Adenosine 5′-Triphosphate Alters Motility and Improves the Fertilizing Capability of Mouse Sperm," Biology of Reproduction 79(1), 164-171, (1 July 2008).
Received: 12 September 2007; Accepted: 1 March 2008; Published: 1 July 2008

This article is only available to subscribers.
It is not available for individual sale.

Get copyright permission
Back to Top