Translator Disclaimer
7 October 2009 Expression and Distribution of NADPH Oxidase Isoforms in Human Myometrium—Role in Angiotensin II-induced Hypertrophy
Xiao-Lan Cui, Baojun Chang, Leslie Myatt
Author Affiliations +
Abstract

The renin-angiotensin system is upregulated in pregnant women and may play a role in myometrial hypertrophy during pregnancy. We examined whether angiotensin II could induce myometrial protein synthesis as determined by 3H-leucine incorporation in an immortalized human myometrial smooth muscle cell line (ULTR cells). The effects of angiotensin II were mediated by NADPH oxidase because diphenylene iodonium abolished angiotensin II-induced protein synthesis. We investigated gene expression and cellular localization of NADPH oxidase isoforms in ULTR cells and confirmed expression of NOX1, NOX4, and NOX5 in myometrial tissue. Angiotensin II induced a cellular redistribution and upregulation of NOX5 protein without altering NOX1 and NOX4 expression. It seems the effect of angiotensin II relies on the type 1 receptor (AT1), because losartan significantly blocked angiotensin II-induced increase in 3H-leucine incorporation. We conclude that NADPH oxidase mediates angiotensin II-stimulated protein synthesis downstream of AT1 in myometrium smooth muscle cells.

Xiao-Lan Cui, Baojun Chang, and Leslie Myatt "Expression and Distribution of NADPH Oxidase Isoforms in Human Myometrium—Role in Angiotensin II-induced Hypertrophy," Biology of Reproduction 82(2), 305-312, (7 October 2009). https://doi.org/10.1095/biolreprod.109.080275
Received: 28 July 2009; Accepted: 1 September 2009; Published: 7 October 2009
JOURNAL ARTICLE
8 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top