Translator Disclaimer
24 February 2010 EPAS1 Is Required for Spermatogenesis in the Postnatal Mouse Testis
Author Affiliations +
Abstract

Spermatogenesis, a process involving the differentiation of spermatogonial stem cells into mature spermatozoa, takes place throughout masculine life. A complex system in the testis, including endocrine signaling, physical interactions between germ and somatic cells, spermatocyte meiosis, and timely release of spermatozoa, controls this cycle. We demonstrate herein that decreased O2 levels and Epas1 activation are critical components of spermatogenesis. Postnatal Epas1 ablation leads to male infertility, with reduced testis size and weight. While immature spermatogonia and spermatocytes are present in Epas1Delta/Delta testes, spermatid and spermatozoan numbers are dramatically reduced. This is not due to germ cell-intrinsic defects. Rather, EpasDelta/Delta Sertoli cells exhibit decreased ability to form tight junctions, thereby disrupting the blood-testis barrier necessary for proper spermatogenesis. Reduced numbers of tight junction complexes are due to decreased expression of multiple genes encoding tight junction proteins, including TJP1 (ZO1), TJP2 (ZO2), and occludin. Furthermore, Epas1Delta/Delta testes exhibit disrupted basement membranes surrounding the seminiferous tubules, causing the premature release of incompletely differentiated germ cells. We conclude that low O2 levels in the male gonad regulate germ cell homeostasis in this organ via EPAS1.

Michaela Gruber, Lijoy K. Mathew, Anja C. Runge, Joseph A. Garcia, and M. Celeste Simon "EPAS1 Is Required for Spermatogenesis in the Postnatal Mouse Testis," Biology of Reproduction 82(6), 1227-1236, (24 February 2010). https://doi.org/10.1095/biolreprod.109.079202
Received: 1 June 2009; Accepted: 1 February 2010; Published: 24 February 2010
JOURNAL ARTICLE
10 PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top