Since the first mouse clone was produced by somatic cell nuclear transfer, the success rate of cloning in mice has been extremely low. Some histone deacetylase inhibitors, such as trichostatin A and scriptaid, have improved the full-term development of mouse clones significantly, but the mechanisms allowing for this are unclear. Here, we found that two other specific inhibitors, suberoylanilide hydroxamic acid and oxamflatin, could also reduce the rate of apoptosis in blastocysts, improve the full-term development of cloned mice, and increase establishment of nuclear transfer-generated embryonic stem cell lines significantly without leading to obvious abnormalities. However, another inhibitor, valproic acid, could not improve cloning efficiency. Suberoylanilide hydroxamic acid, oxamflatin, trichostatin A, and scriptaid are inhibitors for classes I and IIa/b histone deacetylase, whereas valproic acid is an inhibitor for classes I and IIa, suggesting that inhibiting class IIb histone deacetylase is an important step for reprogramming mouse cloning efficiency.
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4 August 2010
Inhibition of Class IIb Histone Deacetylase Significantly Improves Cloning Efficiency in Mice
Tetsuo Ono,
Chong Li,
Eiji Mizutani,
Yukari Terashita,
Kazuo Yamagata,
Teruhiko Wakayama
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Biology of Reproduction
Vol. 83 • No. 6
December 2010
Vol. 83 • No. 6
December 2010
clone
developmental biology
early development
embryo
HDACi
ntES cell
nuclear transfer