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9 March 2011 Manipulation of Estrogen Synthesis Alters MIR202* Expression in Embryonic Chicken Gonads
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Abstract

Tissue-specific patterns of microRNA (miRNA) expression contribute to organogenesis during embryonic development. Using the embryonic chicken gonads as a model for vertebrate gonadogenesis, we previously reported that miRNAs are expressed in a sexually dimorphic manner during gonadal sex differentiation. Being male biased, we hypothesised that up-regulation of microRNA 202* (MIR202*) is characteristic of testicular differentiation. To address this hypothesis, we used estrogen modulation to induce gonadal sex reversal in embryonic chicken gonads and analyzed changes in MIR202* expression. In ovo injection of estradiol-17beta at Embryonic Day 4.5 (E4.5) caused feminization of male gonads at E9.5 and reduced MIR202* expression to female levels. Female gonads treated at E3.5 with an aromatase inhibitor, which blocks estrogen synthesis, were masculinized by E9.5, and MIR202* expression was increased. Reduced MIR202* expression correlated with reduced expression of the testis-associated genes DMRT1 and SOX9, and up-regulation of ovary-associated genes FOXL2 and CYP19A1 (aromatase). Increased MIR202* expression correlated with down-regulation of FOXL2 and aromatase and up-regulation of DMRT1 and SOX9. These results confirm that up-regulation of MIR202* coincides with testicular differentiation in embryonic chicken gonads.

Stephanie C. Bannister, Craig A. Smith, Kelly N. Roeszler, Timothy J. Doran, Andrew H. Sinclair, and Mark L.V. Tizard "Manipulation of Estrogen Synthesis Alters MIR202* Expression in Embryonic Chicken Gonads," Biology of Reproduction 85(1), 22-30, (9 March 2011). https://doi.org/10.1095/biolreprod.110.088476
Received: 21 September 2010; Accepted: 1 March 2011; Published: 9 March 2011
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