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16 November 2011 Protein Restriction to Pregnant Rats Increases the Plasma Levels of Angiotensin II and Expression of Angiotensin II Receptors in Uterine Arteries
Haijun Gao, Uma Yallampalli, Chandra Yallampalli
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Abstract

Whether gestational protein restriction affects the renin-angiotensin system (RAS) in uterine artery remains unknown. In this study, we hypothesized that gestational protein restriction alters the expression of RAS components in uterine artery. In study one, time-scheduled pregnant Sprague Dawley rats were fed a normal or low-protein (LP) diet from Day 3 of pregnancy until they were killed at Days 19 and 22. The uterine arteries were collected and used for gene expression of Ace, Ace2, Agtr1a, Agtr1b, Agtr2, Esr1, and Esr2 by quantitative real-time PCR and/or Western blotting. LP increased plasma levels of angiotensin II in pregnant rats. In the uterine artery, the expressions of Agtr1a, Agtr1b, and Esr1 were increased by LP at Days 19 and 22 of pregnancy, whereas the abundance of AGTR1 and AGTR2 was increased by LP at Day 19 of pregnancy. The expression of Ace2 was not detectable in rat uterine artery. In study two, virgin female rats were ovariectomized and implanted with either 17beta-estradiol (E2), progesterone (P4), both E2 and P4, or placebo pellets until they were killed 7 days later. In rat uterine artery, E2 and P4 reduced the expression of Agtr1a, and E2 increased the expression of Agtr1b and Agtr2, but neither E2 nor P4 regulated the expression of Ace. These results indicate that gestational protein restriction induces an increase in Agtr1 expression in uterine artery, and thus may exacerbate the vasoconstriction to elevated angiotensin II present in maternal circulation, and that female sex hormones also play a role in this process.

Haijun Gao, Uma Yallampalli, and Chandra Yallampalli "Protein Restriction to Pregnant Rats Increases the Plasma Levels of Angiotensin II and Expression of Angiotensin II Receptors in Uterine Arteries," Biology of Reproduction 86(3), 68-, (16 November 2011). https://doi.org/10.1095/biolreprod.111.095844
Received: 23 August 2011; Accepted: 1 November 2011; Published: 16 November 2011
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KEYWORDS
angiotensin I converting enzyme
angiotensin II/angiotensin II receptor
developmental origins of health and disease
gestational protein restriction
nutrition
pregnancy
rat
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