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7 March 2012 Immunolocalization of Adipocytes and Prostaglandin E2 and Its Four Receptor Proteins EP1, EP2, EP3, and EP4 in the Caprine Cervix During Spontaneous Term Labor
Guosheng Gu, Qian Gao, Xuejun Yuan, Libo Huang, Lijiang Ge
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Abstract

The mechanisms of cervical ripening and dilation in mammals remain obscure. Information is lacking about the localization of prostaglandin E2 (PGE2)-producing cells and PGE2 receptors (EP) in intrapartum cervix and whether cervical dilation at parturition is an active process. To reveal these mechanisms, immunolocalization of EP1EP4 (official gene symbols PTGER1–PTGER4) and PGE2-producing cells in caprine cervix during nonpregnancy, pregnancy, and parturition was assayed by immunohistochemistry (IHC); the mRNA expression levels of PTGS2, PTGER2 (EP2), and PTGER4 (EP4) were determined using quantitative PCR; and the existence of adipocytes in the cervix at various stages was demonstrated with Oil Red O staining and IHC of perilipin A. The results suggested that in intrapartum caprine cervix staining of the PGE2 was observed in the overall tissues, for example, blood vessels, canal or glandular epithelia, serosa, circular and longitudinal muscles, and stroma in addition to adipocytes; EP2 was detectable in all the tissues other than glandular epithelia; EP4 was strongly expressed in all the tissues other than serosa; EP1 was detected mainly in arterioles and canal or glandular epithelia; and EP3 was poorly expressed only in stroma, canal epithelia, and circular muscles. Little or no expression of EP2, EP3, and EP4 as well as PGE2 in all cervical tissues was observed during nonpregnancy and pregnancy except for the strong expression of EP1 in canal or glandular epithelia during pregnancy. The mRNA expression levels of PTGS2, PTGER2, and PTGER4 were significantly higher in intrapartum than nonpregnant and midpregnant cervices (P < 0.01). Adipocytes appear only in the intrapartum cervix. These results support the concept that PGE2 modulates specific functions in various anatomical structures of the caprine cervix at labor and the appearance of adipocytes at labor is likely related to caprine cervical dilation.

© 2012 by the Society for the Study of Reproduction, Inc.
Guosheng Gu, Qian Gao, Xuejun Yuan, Libo Huang, and Lijiang Ge "Immunolocalization of Adipocytes and Prostaglandin E2 and Its Four Receptor Proteins EP1, EP2, EP3, and EP4 in the Caprine Cervix During Spontaneous Term Labor," Biology of Reproduction 86(5), (7 March 2012). https://doi.org/10.1095/biolreprod.111.096040
Received: 27 August 2011; Accepted: 1 February 2012; Published: 7 March 2012
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KEYWORDS
Adipocytes
cervix
EP
goat
parturition
PGE2
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