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29 February 2012 Translation of incenp During Oocyte Maturation Is Required for Embryonic Development in Xenopus laevis
Geoffrey G. Leblond, Heather Sarazin, Ruizhen Li, Makoto Suzuki, Naoto Ueno, X. Johné Liu
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Abstract

The chromosome passenger complex (CPC) consists of Aurora-B kinase and several other subunits. One of these, incenp, binds Aurora-B and regulates its kinase activity. During Xenopus oocyte maturation, incenp accumulates through translation, contributing to aurora-b activation. A previous study has demonstrated that inhibition of incenp translation during oocyte maturation diminishes aurora-b activation but does not interfere with oocyte maturation, characterized by normal maturation-specific cyclin-b phosphorylation, degradation, and resynthesis. Here we have extended these findings, showing that inhibition of incenp translation during oocyte maturation did not interfere with meiosis I or II, as indicated by the normal emission of the first polar body and metaphase II arrest, followed by the successful emission of the second polar body upon parthenogenetic egg activation. Most importantly, however, when transferred to host frogs and subsequently ovulated, the incenp-deficient eggs were fertilized but failed to undergo mitotic cleavage. Thus, translation of incenp during oocyte maturation appears to be part of oocyte cytoplasmic maturation, preparing the egg for the rapid mitosis following fertilization.

© 2012 by the Society for the Study of Reproduction, Inc.
Geoffrey G. Leblond, Heather Sarazin, Ruizhen Li, Makoto Suzuki, Naoto Ueno, and X. Johné Liu "Translation of incenp During Oocyte Maturation Is Required for Embryonic Development in Xenopus laevis," Biology of Reproduction 86(5), (29 February 2012). https://doi.org/10.1095/biolreprod.111.097972
Received: 26 November 2011; Accepted: 1 February 2012; Published: 29 February 2012
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KEYWORDS
cytoplasmic maturation
embryo cleavage
host-transfer
incenp
translation
Xenopus laevis
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