Translator Disclaimer
18 April 2012 Sphingomyelinase Activity in Mother's Milk Is Essential for Juvenile Development: A Case from Lactating Tsetse Flies
Joshua B. Benoit, Geoffrey M. Attardo, Veronika Michalkova, Peter Takáč, Jana Bohova, Serap Aksoy
Author Affiliations +
Abstract

Sphingosine is a structural component of sphingolipids. The metabolism of phosphoethanolamine ceramide (sphingomyelin) by sphingomyelinase (SMase), followed by the breakdown of ceramide by ceramidase (CDase) yields sphingosine. Female tsetse fly is viviparous and generates a single progeny within her uterus during each gonotrophic cycle. The mother provides her offspring with nutrients required for development solely via intrauterine lactation. Quantitative PCR showed that acid smase1 (asmase1) increases in mother's milk gland during lactation. aSMase1 was detected in the milk gland and larval gut, indicating this protein is generated during lactation and consumed by the larva. The higher levels of SMase activity in larval gut contents indicate that this enzyme is activated by the low gut pH. In addition, cdase is expressed at high levels in the larval gut. Breakdown of the resulting ceramide is likely accomplished by the larval gut-secreted CDase, which allows absorption of sphingosine. We used the tsetse system to understand the critical role(s) of SMase and CDase during pregnancy and lactation and their downstream effects on adult progeny fitness. Reduction of asmase1 by short interfering RNA negatively impacted pregnancy and progeny performance, resulting in a 4–5-day extension in pregnancy, 10%–15% reduction in pupal mass, lower pupal hatch rates, impaired heat tolerance, reduced symbiont levels, and reduced fecundity of adult progeny. This study suggests that the SMase activity associated with tsetse lactation and larval digestion is similar in function to that of mammalian lactation and represents a critical process for juvenile development, with important effects on the health of progeny during their adulthood.

© 2012 by the Society for the Study of Reproduction, Inc.
Joshua B. Benoit, Geoffrey M. Attardo, Veronika Michalkova, Peter Takáč, Jana Bohova, and Serap Aksoy "Sphingomyelinase Activity in Mother's Milk Is Essential for Juvenile Development: A Case from Lactating Tsetse Flies," Biology of Reproduction 87(1), (18 April 2012). https://doi.org/10.1095/biolreprod.112.100008
Received: 17 February 2012; Accepted: 1 April 2012; Published: 18 April 2012
JOURNAL ARTICLE
PAGES

This article is only available to subscribers.
It is not available for individual sale.
+ SAVE TO MY LIBRARY

SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top