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3 June 2015 Rbbp7 Is Required for Uterine Stromal Decidualization in Mice
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Abstract

Uterine stromal cells undergo extensive proliferation and differentiation during postimplantation development, a process known as decidualization. While a range of signaling molecules have been demonstrated to play essential roles in this event, its potential epigenetic regulatory mechanisms remain largely unknown. Retinoblastoma binding protein 7 (Rbbp7) is a protein reported as a core component of many histone modification and chromatin remodeling complexes. In the present study, our in situ hybridization and immunochemistry analysis first reveals a spatiotemporal expression of Rbbp7 in the uterus during the peri-implantation period. Observations of remarkable induction of Rbbp7 expression in uterine stromal cells in response to progesterone-nuclear receptor PR signaling point to its potential physiological significance during postimplantation uterine development. Employing a stealth RNA knockdown approach, combined with primary murine uterine stromal cell culture and an in vitro-induced decidualization model, we further demonstrate that Rbbp7 silencing compromises stromal cell decidualization via attenuating histone H4 acetylation and cyclin D3 expression. The results collectively suggest that Rbbp7 is a potentially functional player regulating normal histone acetylation modification and cyclin D3 expression in stromal cells during postimplantation decidual development.

Hui He, Shuangbo Kong, Fei Liu, Shuang Zhang, Yaling Jiang, Yixin Liao, Yufei Jiang, Qian Li, Bingyan Wang, Zuomin Zhou, Haibin Wang, and Ran Huo "Rbbp7 Is Required for Uterine Stromal Decidualization in Mice," Biology of Reproduction 93(1), (3 June 2015). https://doi.org/10.1095/biolreprod.115.129015
Received: 9 February 2015; Accepted: 1 May 2015; Published: 3 June 2015
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