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19 November 2015 Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice
Masanaga Muto, Yoshitaka Fujihara, Tomohiro Tobita, Daiji Kiyozumi, Masahito Ikawa
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Abstract

The X-linked Plac1 gene is maternally expressed in trophoblast cells during placentation, and its disruption causes placental hyperplasia and intrauterine growth restriction. In contrast, Plac1 is also reported to be one of the upregulated genes in the hyperplastic placenta generated by nuclear transfer. However, the effect of overexpressed Plac1 on placental formation and function remained unaddressed. We complemented the Plac1 knockout placental dysfunction by lentiviral vector-mediated, placenta-specific Plac1 transgene expression. Whereas fetal development and the morphology of maternal blood sinuses in the labyrinth zone improved, placental hyperplasia remained, with an expanded the junctional zone that migrated and encroached into the labyrinth zone. Further experiments revealed that wild-type placenta with transgenically expressed Plac1 resulted in placental hyperplasia without the encroaching of the junctional zone. Our findings suggest that Plac1 is involved in trophoblast cell proliferation, differentiation, and migration. Its proper expression is required for normal placentation and fetal development.

© 2016 by the Society for the Study of Reproduction, Inc.
Masanaga Muto, Yoshitaka Fujihara, Tomohiro Tobita, Daiji Kiyozumi, and Masahito Ikawa "Lentiviral Vector-Mediated Complementation Restored Fetal Viability but Not Placental Hyperplasia in Plac1-Deficient Mice," Biology of Reproduction 94(1), (19 November 2015). https://doi.org/10.1095/biolreprod.115.133454
Received: 15 July 2015; Accepted: 1 November 2015; Published: 19 November 2015
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