Translator Disclaimer
20 December 2016 Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle
Benjamin Sigurgeirsson, Hanna Åmark, Anders Jemt, Dorina Ujvari, Magnus Westgren, Joakim Lundeberg, Sebastian Gidlöf
Author Affiliations +
Abstract

Endometrial receptivity is crucial for implantation and establishment of a normal pregnancy. The shift from proliferative to receptive endometrium is still far from being understood. In this paper, we comprehensively present the transcriptome of the human endometrium by comparing endometrial biopsies from proliferative phase with consecutive biopsies 7–9 days after ovulation. The results show a clear difference in expression between the two time points using both total and small RNA sequencing. A total of 3,297 messenger RNAs (mRNAs), 516 long noncoding RNAs (lncRNAs), and 102 small noncoding RNAs were identified as statistically differentially expressed between the two time points. We show a thorough description of the change in mRNA between the two time points and display lncRNAs, small nucleolar RNAs, and small nuclear RNAs not previously reported in the healthy human endometrium. In conclusion, this paper reports in detail the shift in RNA expression from the proliferative to receptive endometrium.

Summary Sentence

Messenger RNA, sncRNA, and lncRNA show a clear difference in expression between proliferative phase and 7–9 days after ovulation, thoroughly described together with lncRNA, snoRNA, and snRNA not previously reported in healthy human endometrium.

© The Authors 2016. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please journals.permissions@oup.com
Benjamin Sigurgeirsson, Hanna Åmark, Anders Jemt, Dorina Ujvari, Magnus Westgren, Joakim Lundeberg, and Sebastian Gidlöf "Comprehensive RNA sequencing of healthy human endometrium at two time points of the menstrual cycle," Biology of Reproduction 96(1), 24-33, (20 December 2016). https://doi.org/10.1095/biolreprod.116.142547
Received: 15 June 2016; Accepted: 10 November 2016; Published: 20 December 2016
JOURNAL ARTICLE
10 PAGES


Share
SHARE
ARTICLE IMPACT
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top