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17 January 2017 Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA–mRNA interactome
Ashley F. George, Kathleen M. Rahman, Meredith E. Camp, Nripesh Prasad, Frank F. Bartol, Carol A. Bagnell
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Abstract

Factors delivered to offspring in colostrum within 2 days of birth support neonatal porcine uterine development. The uterine mRNA transcriptome is affected by age and nursing during this period. Whether uterine microRNA (miRNA) expression is affected similarly is unknown. Objectives were to (1) determine effects of age and nursing on porcine uterine miRNA expression between birth and postnatal day (PND) 2 using miRNA sequencing (miRNAseq) and; (2) define affected miRNA–mRNA interactions and associated biological processes using integrated target prediction analysis.At birth (PND 0), gilts were euthanized, nursed ad libitum, or gavage-fed milk replacer for 48 h. Uteri were collected at birth or 50 h postnatal. MicroRNAseq data were validated using quantitative real-time PCR. Targets were predicted using an established RNA database generated from the same tissues. For PND 2 versus PND 0 comparisons, 31 differentially expressed (DE) miRNAs were identified for nursed, and 42 DE miRNAs were identified for replacer-fed gilts. Six DE miRNAs were identified for nursed versus replacer-fed gilts on PND 2. Target prediction for inversely correlated DE miRNA–mRNA pairings indicated 20 miRNAs targeting 251 mRNAs in nursed, versus 29 miRNAs targeting 585 mRNAs in replacer-fed gilts for PND 2 versus PND 0 comparisons, and 5 miRNAs targeting 81 mRNAs for nursed versus replacer-fed gilts on PND 2. Biological processes predicted to be affected by age and nursing included cell-to-cell signaling, cell morphology, and tissue morphology. Results indicate novel age- and lactocrine-sensitive miRNA–mRNA relationships associated with porcine neonatal uterine development between birth and PND 2.

Summary Sentence

Comprehensive microRNA–mRNA analyses identified novel age- and lactocrine-sensitive porcine uterine microRNAs, microRNA–mRNA interactions, and biological processes associated with porcine neonatal uterine development.

©The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/bync/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
Ashley F. George, Kathleen M. Rahman, Meredith E. Camp, Nripesh Prasad, Frank F. Bartol, and Carol A. Bagnell "Defining age- and lactocrine-sensitive elements of the neonatal porcine uterine microRNA–mRNA interactome," Biology of Reproduction 96(2), 327-340, (17 January 2017). https://doi.org/10.1093/biolre/iox001
Received: 11 November 2016; Accepted: 10 January 2017; Published: 17 January 2017
JOURNAL ARTICLE
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