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27 April 2017 Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein a complex and DNA double-strand breaks
Yang Xu, Roger A. Greenberg, Ernst Schonbrunn, P. Jeremy Wang
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Abstract

Meiotic recombination ensures faithful segregation of homologous chromosomes during meiosis and generates genetic diversity in gametes. MEIOB (meiosis specific with OB domains), a meiosis specific single-stranded DNA-binding homolog of replication protein A1 (RPA1), is essential for meiotic recombination. Here, we investigated the molecular mechanisms of MEIOB by characterizing its binding partners spermatogenesis associated 22 (SPATA22) and RPA. We find that MEIOB and SPATA22 form an obligate complex and contain defined interaction domains. The interaction between these two proteins is unusual in that nearly any deletion in the binding domains abolishes the interaction. Strikingly, a single residue D383 in MEIOB is indispensable for the interaction. The MEIOB/SPATA22 complex interacts with the RPA heterotrimeric complex in a collaborative manner. Furthermore, MEIOB and SPATA22 are recruited to induced DNA double-strand breaks (DSBs) together but not alone. These results demonstrate the cooperative property of the MEIOB-SPATA22 complex in its interaction with RPA and recruitment to DSBs.

Summary Sentence

MEIOB and SPATA22 function as a novel meiosis-specific heterocomplex.

© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please journals.permissions@oup.com
Yang Xu, Roger A. Greenberg, Ernst Schonbrunn, and P. Jeremy Wang "Meiosis-specific proteins MEIOB and SPATA22 cooperatively associate with the single-stranded DNA-binding replication protein a complex and DNA double-strand breaks," Biology of Reproduction 96(5), 1096-1104, (27 April 2017). https://doi.org/10.1093/biolre/iox040
Received: 15 February 2017; Accepted: 26 April 2017; Published: 27 April 2017
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