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30 May 2017 Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion
Ahmed Z. Balboula, Cecilia S. Blengini, Amanda S. Gentilello, Masashi Takahashi, Karen Schindler
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Abstract

During oocyte meiotic maturation, Aurora kinase C (AURKC) is required to accomplish many critical functions including destabilizing erroneous kinetochore–microtubule (K-MT)attachments and regulating bipolar spindle assembly. How localized activity of AURKC is regulated in mammalian oocytes, however, is not fully understood. Female gametes from many species, including mouse, contain stores of maternal transcripts that are required for downstream developmental events. We show here that depletion of maternal RNA in mouse oocytes resulted in impaired meiotic progression, increased incidence of chromosome misalignment and abnormal spindle formation at metaphase I (Met I), and cytokinesis defects. Importantly, depletion of maternal RNA perturbed the localization and activity of AURKC within the chromosomal passenger complex (CPC). These perturbations were not observed when translation was inhibited by cycloheximide (CHX) treatment. These results demonstrate a translation-independent function of maternal RNA to regulate AURKC-CPC function in mouse oocytes.

Summary Sentence

Maternal RNA contained in mouse oocytes regulates localized AURKC-CPC activity independent of its role in translation to support meiotic maturation.

© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Ahmed Z. Balboula, Cecilia S. Blengini, Amanda S. Gentilello, Masashi Takahashi, and Karen Schindler "Maternal RNA regulates Aurora C kinase during mouse oocyte maturation in a translation-independent fashion," Biology of Reproduction 96(6), 1197-1209, (30 May 2017). https://doi.org/10.1093/biolre/iox047
Received: 15 November 2016; Accepted: 26 May 2017; Published: 30 May 2017
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