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13 October 2017 A teleost androgen promotes development of primary ovarian follicles in coho salmon and rapidly alters the ovarian transcriptome
Christopher Monson, Kristy Forsgren, Giles Goetz, Louisa Harding, Penny Swanson, Graham Young
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Abstract

Recent studies using several teleost models have revealed that androgens increase the size of previtellogenic (primary and/or early secondary) ovarian follicles. To explore our hypothesis that androgens drive the development of primary follicles into early secondary follicles, and to determine the mechanisms underlying these androgenic effects, we exposed juvenile coho salmon to nearphysiological and relatively sustained levels of the nonaromatizable androgen 11-ketotestosterone (11-KT). This resulted in significant growth of primary ovarian follicles after 10 and 20 days, with follicles after 20 days displaying a morphological phenotype characteristic of early secondary follicles (presence of cortical alveoli). Utilizing the same experimental approach, we then analyzed how 11-KT rapidly altered the ovarian transcriptome after 1 and 3 days of treatment. RNA-Seq analysis revealed that 69 (day 1) and 1,022 (day 3) contiguous sequences (contigs) were differentially expressed relative to controls. The differentially expressed contigs mapped to genes including those encoding proteins involved in gonadotropin, steroid hormone, and growth factor signaling, and in cell and ovarian development, including genes with putative androgen-response elements. Biological functions and canonical pathways identified as potentially altered by 11-KT include those involved in ovarian development, tissue differentiation and remodeling, and lipid metabolism. We conclude that androgens play amajor role in stimulating primary ovarian follicle development and the transition into secondary growth.

Summary Sentence

In vivo treatment of coho salmon with the androgen, 11-ketotestosterone, dramatically alters the ovarian transcriptome during primary oocyte growth, and promotes the completion of primary ovarian follicle growth.

© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Christopher Monson, Kristy Forsgren, Giles Goetz, Louisa Harding, Penny Swanson, and Graham Young "A teleost androgen promotes development of primary ovarian follicles in coho salmon and rapidly alters the ovarian transcriptome," Biology of Reproduction 97(5), 731-745, (13 October 2017). https://doi.org/10.1093/biolre/iox124
Received: 22 May 2017; Accepted: 12 October 2017; Published: 13 October 2017
JOURNAL ARTICLE
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KEYWORDS
11-ketotestosterone
Androgens
follicular development
gene expression
ovarian follicles
ovarian transcriptome
teleost
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