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30 October 2017 Germline competency of human embryonic stem cells depends on eomesodermin
Di Chen, Wanlu Liu, Anastasia Lukianchikov, Grace V. Hancock, Jill Zimmerman, Matthew G. Lowe, Rachel Kim, Zoran Galic, Naoko Irie, M. Azim Surani, Steven E. Jacobsen, Amander T. Clark
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Abstract

In humans, germline competency and the specification of primordial germ cells (PGCs) are thought to occur in a restricted developmental window during early embryogenesis. Despite the importance of specifying the appropriate number of PGCs for human reproduction, the molecular mechanisms governing PGC formation remain largely unexplored. Here, we compared PGC-like cell (PGCLC) differentiation from 18 independently derived human embryonic stem cell (hESC) lines, and discovered that the expression of primitive streak genes were positively associated with hESC germline competency. Furthermore, we show that chemical inhibition of TGFβ and WNT signaling, which are required for primitive streak formation and CRISPR/Cas9 deletion of Eomesodermin (EOMES), significantly impacts PGCLC differentiation from hESCs. Taken together, our results suggest that human PGC formation involves signaling and transcriptional programs associated with somatic germ layer induction and expression of EOMES.

Summary Sentence

EOMES induction in the progenitor cell prior to germ cell formation in vitro from hESCs is required for efficient PGC-like cell formation.

© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Di Chen, Wanlu Liu, Anastasia Lukianchikov, Grace V. Hancock, Jill Zimmerman, Matthew G. Lowe, Rachel Kim, Zoran Galic, Naoko Irie, M. Azim Surani, Steven E. Jacobsen, and Amander T. Clark "Germline competency of human embryonic stem cells depends on eomesodermin," Biology of Reproduction 97(6), 850-861, (30 October 2017). https://doi.org/10.1093/biolre/iox138
Received: 23 October 2017; Accepted: 27 October 2017; Published: 30 October 2017
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KEYWORDS
Embryonic stem cells
EOMES
human
primordial germ cells
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