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8 November 2017 Dimethyl sulfoxide and quercetin prolong the survival, motility, and fertility of cold-stored mouse sperm for 10 days
Hidetaka Yoshimoto, Toru Takeo, Naomi Nakagata
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Technology for preserving sperm is useful for disseminating valuable male genetic traits. Cold storage is suitable for easily transporting sperm as an alternative to the shipment of live animals. However, there is a technical limitation in that the fertility of cold-stored sperm declines within 3 days. To overcome this problem, we examined the protective effects of quercetin and dimethyl sulfoxide (DMSO). DMSO and quercetin maintained the fertility and motility of cold-stored sperm for 10 days. In addition, quercetin attenuated the reduction of mitochondrial membrane potential of cold-stored sperm during sperm preincubation, allowing the induction of capacitation, and it localized to the midpiece of sperm. Furthermore, DMSO and quercetin enhanced the level of tyrosine phosphorylation of cold-stored sperm. DMSO and quercetin have life-prolonging effects on sperm during cold storage. Cold storage using DMSO and quercetin will provide a robust system for internationally transporting valuable sperm samples.

Summary Sentence

Cold storage of mouse sperm with dimethyl sulfoxide and quercetin achieved the longest period to produce two-cell embryos by in vitro fertilization.

© The Author(s) 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact
Hidetaka Yoshimoto, Toru Takeo, and Naomi Nakagata "Dimethyl sulfoxide and quercetin prolong the survival, motility, and fertility of cold-stored mouse sperm for 10 days," Biology of Reproduction 97(6), 883-891, (8 November 2017).
Received: 1 September 2017; Accepted: 7 November 2017; Published: 8 November 2017

cold storage
dimethyl sulfoxide
tyrosine phosphorylation
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