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12 January 2018 Meiotic spindle formation in mammalian oocytes: implications for human infertility
Suk Namgoong, Nam-Hyung Kim
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Abstract

In the final stage of oogenesis, mammalian oocytes generate a meiotic spindle and undergo chromosome segregation to yield an egg that is ready for fertilization. Herein, we describe the recent advances in understanding the mechanisms controlling formation of the meiotic spindle in metaphase I (MI) and metaphase II (MII) in mammalian oocytes, and focus on the differences between mouse and human oocytes. Unlikemitotic cells, mammalian oocytes lack typical centrosomes that consist of two centrioles and the surrounding pericentriolar matrix proteins, which serve as microtubule-organizing centers (MTOCs) inmost somatic cells. Instead, oocytes rely on different mechanisms for the formation of microtubules in MI spindles. Two different mechanisms have been described for MI spindle formation in mammalian oocytes. Chromosome-mediated microtubule formation, including RAN-mediated spindle formation and chromosomal passenger complex-mediated spindle elongation, controls the growth of microtubules from chromatin, while acentriolar MTOC-mediated microtubule formation contributes to spindle formation. Mouse oocytes utilize both chromatin- and MTOC-mediated pathways for microtubule formation. The existence of both pathways may provide a fail-safe mechanism to ensure high fidelity of chromosome segregation during meiosis. Unlike mouse oocytes, human oocytes considered unsuitable for clinical in vitro fertilization procedures, lack MTOCs; this may explain why meiosis in human oocytes is often error-prone. Understanding the mechanisms of MI/MII spindle formation, spindle assembly checkpoint, and chromosome segregation, in mammalian oocytes, will provide valuable insights into the molecular mechanisms of human infertility.

Summary Sentence

Recent finding in meiotic spindle formations of mammalian oocyte uncover new insights into the molecular mechanism of human infertility.

© The Authors 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Suk Namgoong and Nam-Hyung Kim "Meiotic spindle formation in mammalian oocytes: implications for human infertility," Biology of Reproduction 98(2), 153-161, (12 January 2018). https://doi.org/10.1093/biolre/iox145
Received: 4 July 2017; Accepted: 27 December 2017; Published: 12 January 2018
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KEYWORDS
aneuploidy
meiosis
microtubule-organization centers
oocyte maturation
spindle
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