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13 February 2018 CREB3 regulatory factor -mTOR-autophagy regulates goat endometrial function during early pregnancy
Diqi Yang, Tingting Jiang, Jianguo Liu, Beibei Zhang, Pengfei Lin, Huatao Chen, Dong Zhou, Keqiong Tang, Aihua Wang, Yaping Jin
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Abstract

In domestic ruminants, a receptive endometrium is crucial for successful pregnancy. Although many essential molecular modulators and pathways have been identified during early pregnancy, the precise mechanisms regulating goat endometrial function remains largely unknown. Here, we describe a novel regulator during early pregnancy, whereby hormones increased CREB3 regulatory factor (CREBRF) expression and act as a potential activator of autophagy in endometrial epithelial cells (EECs) via the mTOR pathway. Our results showed that knockdown of CREBRF via shCREBRF hampered EECs proliferation by S-phase cell cycle arrest and significantly inhibited endometrial function. We also reported that CREBRF-mTOR-autophagy pathway plays a vital role in regulating endometrial function, with a blockade of the mTOR by rapamycin demonstrating the regulatory function on prostaglandin (PGs) secretion and cell attachment in EECs. Moreover, chloroquine pretreatment also proved the above conclusion. Collectively, our findings provide new insight into the molecular mechanisms of goat endometrial function and indicate that the CREBRF-mTORautophagy pathway plays a central role in PGs secretion and cell attachment.

Summary Sentence

The protein CREB3 regulatory factor (CREBRF) acts as a potential activator of autophagy in endometrial epithelial cells via the mTOR pathway.Knockdown of CREBRF via shCREBRF hampered EECs proliferation by S-phase cell cycle arrest and significantly inhibited endometrial function. The CREBRF-mTOR-autophagy pathway plays a vital role in regulating endometrial function, with a blockade of the mTOR by rapamycin demonstrating the regulatory function on prostaglandins (PGs) secretion and cell attachment in EECs.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Diqi Yang, Tingting Jiang, Jianguo Liu, Beibei Zhang, Pengfei Lin, Huatao Chen, Dong Zhou, Keqiong Tang, Aihua Wang, and Yaping Jin "CREB3 regulatory factor -mTOR-autophagy regulates goat endometrial function during early pregnancy," Biology of Reproduction 98(5), 713-721, (13 February 2018). https://doi.org/10.1093/biolre/ioy044
Received: 29 November 2017; Accepted: 12 February 2018; Published: 13 February 2018
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