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20 December 2017 Effects of active immunization with newly modified GnRH peptides on spermatogenesis and production performance of Holstein bulls
Mengsi Xu, Chunsheng Xu, Fuyuan Liu, Xiaoyun Shen, Jimeng Meng, Hongli Chen, Jinquan Yang, Ping Zhou, Rui Gao, Shangquan Gan
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Abstract

Immunocastration via vaccination against gonadotropin-releasing hormone (GnRH) is an effective alternative to surgical castration in livestock. In this study, male mice were immunized with eight GnRH peptide derivatives. Two, which exhibited highly significant effects in mice, and one which exhibited the least significant effects were selected for active immunization of 13-month-old bulls. The effects of these GnRH vaccines on sexual development and meat quality in bulls were evaluated by examining testis length, serum hormone and GnRH antibody concentrations, observation of sexual behavior and testicular tissue sections, and evaluation of meat quality indexes. The results indicated that anti-GnRH titers increased rapidly (P < 0.05) and serum follicle stimulating hormone, luteinizing hormone, and testosterone concentrations decreased sharply after booster immunization (P < 0.05), while testis volumes were lower (P < 0.01), testicular growth was arrested and spermatogenesis inhibited in group C GnRH-treated versus control bull groups. Meat quality was not significantly different in immunocastrates relative to bulls in the control group. Our collective results provide a scientific basis to further clarify the mechanisms underlying GnRH-mediated regulation of livestock reproduction, and contribute to the development of an efficient, safe, and reversible immune castration vaccine.

Summary Sentence

Studying active immunization against GnRH has been a focus for future research. However, the immunogenicity of GnRH monomeric derivatives were produced undesirable results. In this paper, we successfully developed a highly efficient animal castration vaccine.

© The Author(s) 2017. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Mengsi Xu, Chunsheng Xu, Fuyuan Liu, Xiaoyun Shen, Jimeng Meng, Hongli Chen, Jinquan Yang, Ping Zhou, Rui Gao, and Shangquan Gan "Effects of active immunization with newly modified GnRH peptides on spermatogenesis and production performance of Holstein bulls ," Biology of Reproduction 99(2), 461-472, (20 December 2017). https://doi.org/10.1093/biolre/iox176
Received: 17 August 2017; Accepted: 18 December 2017; Published: 20 December 2017
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