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28 February 2018 Localization of dimethylated histone three lysine four in the Rattus norvegicus sperm genome
Shelby Wilson, Edward Dere, David Klein, Christoph Schorl, Susan Hall, Kim Boekelheide
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Abstract

We examined the precise localization of dimethylated histone three lysine four (H3K4me2) in mature rat sperm. Within nonintergenic-enriched regions, half of the DNA peaks associated with H3K4me2 retention fell in gene bodies and the other half in promoter regions. The most significant peaks near annotated DNA regions in the composite data included loci known to be associated with RNA metabolism, cell cycle regulation, and spermatogenesis. Regions associated with differential retention of H3K4me2 within gene bodies were significantly enriched for housekeeping gene and cell-cycle functionality. Proximal promoter-associated peaks were enriched for viral reproduction and cell cycle regulation genes, while Promoter1k and Promoter3k peaks were enriched for RNA metabolism functions. Further, homeobox- and kruppel-like factor motifs were among the most significantly enriched de novo and known motifs discovered within gene-associated H3K4me2 peaks. Motif analysis and native chromatin immunoprecipitation followed by sequencing (nChIPseq) peak calling indicated an instructive role for retained paternal histones in the epigenetic regulation of early embryonic development in the rat.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Shelby Wilson, Edward Dere, David Klein, Christoph Schorl, Susan Hall, and Kim Boekelheide "Localization of dimethylated histone three lysine four in the Rattus norvegicus sperm genome ," Biology of Reproduction 99(2), 266-268, (28 February 2018). https://doi.org/10.1093/biolre/ioy051
Received: 27 December 2017; Accepted: 26 February 2018; Published: 28 February 2018
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