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13 March 2018 Hypoxia-inducible factor-1α-dependent autophagy plays a role in glycolysis switch in mouse granulosa cells
Jilong Zhou, Chengyu Li, Wang Yao, M. C Alsiddig, Lijun Huo, Honglin Liu, Yi-Liang Miao
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Abstract

Autophagy is an essential cellularmechanism that degrades cytoplasmic proteins and organelles to recycle their components. Here we showed that autophagy was essential for the glycolysis switch and energy homeostasis in mouse granulosa cells under hypoxic condition. Our data indicated that hypoxia inducible factor-1α (HIF-1α) could be largely increased in developing follicles and this remarkable upregulation of HIF-1α triggered cell autophagy and glucose uptake. We found that blocking autophagy by Atg7 knockdown and 3-methyladenine (3-MA) treatment affected the glucose metabolism, with increased glycolytic enzyme activity and decreased ATP production. We also found enhanced lactate level, which was harmful to granulosa cells and could induce cell apoptosis. Thus, our findings highlight a protective role of HIF-1α-dependent autophagy for the granulosa cell glycolysis switch in both energy supply and cell survival.

Summary Sentence

HIF-1α-induced autophagy is essential for glycolysis metabolism in mouse granulosa cells.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Jilong Zhou, Chengyu Li, Wang Yao, M. C Alsiddig, Lijun Huo, Honglin Liu, and Yi-Liang Miao "Hypoxia-inducible factor-1α-dependent autophagy plays a role in glycolysis switch in mouse granulosa cells ," Biology of Reproduction 99(2), 308-318, (13 March 2018). https://doi.org/10.1093/biolre/ioy061
Received: 24 November 2017; Accepted: 9 March 2018; Published: 13 March 2018
JOURNAL ARTICLE
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