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5 April 2018 Sex steroids drive the remodeling of oviductal extracellular matrix in cattle
Angela Maria Gonella-Diaza, Fernando Silveira Mesquita, Everton Lopes, Kauê Ribeiro da Silva, Bruno Cogliati, Ricardo De Francisco Strefezzi, Mario Binelli
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Abstract

The extracellular matrix (ECM) is a group of molecules that offer structural and biochemical support to cells and interact with them to regulate their function. Also, growth factors (GFs) stored in the ECM can be locally released during ECM remodeling. Here, we hypothesize that the balance between ECM components and remodelers is regulated according to the ovarian steroid milieu to which the oviduct is exposed during the periovulatory period. Follicular growth was manipulated to generate cows that ovulated small follicles (SF-small corpus luteum [SCL]; n = 20) or large follicles (LF-large corpus luteum [LCL]; n = 21) and possess corresponding Estradiol (E2) and Progesterone (P4) plasmatic concentrations. Ampulla and isthmus samples were collected on day 4 (day 0 = ovulation induction) and immediately frozen or fixed. The transcriptional profile (n = 3/group) was evaluated by RNA sequencing. MMP Antibody Array was used to quantify ECM remodelers' protein abundance and immunohistochemistry to quantify type I collagen. Transcriptome analysis revealed the over-representation of ECM organization and remodeling pathways in the LF-LCL group. Transcription of ECM components (collagens), remodelers (ADAMs and MMPs), and related GFs were upregulated in LF-LCL. Protein intensities for MMP3, MMP8, MMP9, MMP13, and TIMP4 were greater for the LF-LCL group. Type I collagen content in the mucosa was greater in SF-SCL group. In conclusion, that the earlier and more intense exposure to E2 and P4 during the periovulatory period in LF-LCL animals stimulates ECM remodeling. We speculate that differential ECM regulation may contribute to oviductal receptivity to the embryo.

Summary Sentence

Size of preovulatory follicle regulates ECM machinery abundance and function in the oviduct.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Angela Maria Gonella-Diaza, Fernando Silveira Mesquita, Everton Lopes, Kauê Ribeiro da Silva, Bruno Cogliati, Ricardo De Francisco Strefezzi, and Mario Binelli "Sex steroids drive the remodeling of oviductal extracellular matrix in cattle," Biology of Reproduction 99(3), 590-599, (5 April 2018). https://doi.org/10.1093/biolre/ioy083
Received: 5 February 2018; Accepted: 4 April 2018; Published: 5 April 2018
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