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17 April 2018 Effects of preovulatory estradiol on uterine environment and conceptus survival from fertilization to maternal recognition of pregnancy
Emmalee J. Northrop, Jerica J. J. Rich, Robert A. Cushman, Anthony K. McNeel, Émerson M. Soares, Kelsey Brooks, Thomas E. Spencer, George A. Perry
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Abstract

Preovulatory estradiol is known to impact embryo quality and survival. The objective of this study was to determine the effects of preovulatory estradiol on the uterine environment and conceptus survival through maternal recognition of pregnancy. Beef cows/heifers were AIed following induced ovulation. Cows were grouped into high and low preovulatory estradiol. Conceptuses were collected on day 16 nonsurgically (Rep 1; n = 20), or following slaughter (Rep 2; n = 29). Blood was collected to determine plasma glucose concentrations, and uterine luminal fluid (ULF) was analyzed for protein, glucose, and interferon tau (IFNT) concentrations. Total cellular RNA was extracted from caruncular (CAR) and intercaruncular (INCAR) endometrial tissue. There was no effect of preovulatory estradiol on conceptus recovery rate (P = 0.38) or on apoptosis rate in the trophectoderm (P = 0.64). Cows in which a conceptus was recovered had greater concentrations of protein in the ULF (P = 0.04). Animals with elevated preovulatory estradiol had greater endometrial abundance of SLC2A1 (P = 0.05) and SLC5A1 (P = 0.04) in both INCAR and CAR tissue. Presence of a conceptus also tended to increase (P = 0.10) abundance of SLC5A1 in INCAR. In CAR tissue, cows with a conceptus had decreased SLC2A4 abundance (P = 0.05). In summary, conceptus recovery rates, apoptosis in the trophectoderm, IFNT, glucose, and protein concentration in ULF did not differ between cows that did or did not have an increase in preovulatory estradiol concentrations. Thus, there is no indication of increased conceptus survival to day 16 of pregnancy based on estradiol concentrations.

Summary Sentence

Conceptus survival to d 16 was similar among cows with and without elevated preovulatory estradiol; however, glucose transporter expression, and glucose and protein concentration in uterine fluids were influenced by estradiol and conceptus presence.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Emmalee J. Northrop, Jerica J. J. Rich, Robert A. Cushman, Anthony K. McNeel, Émerson M. Soares, Kelsey Brooks, Thomas E. Spencer, and George A. Perry "Effects of preovulatory estradiol on uterine environment and conceptus survival from fertilization to maternal recognition of pregnancy," Biology of Reproduction 99(3), 629-638, (17 April 2018). https://doi.org/10.1093/biolre/ioy086
Received: 18 September 2017; Accepted: 15 April 2018; Published: 17 April 2018
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