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16 April 2018 Restraint-induced corticotrophin-releasing hormone elevation triggers apoptosis of ovarian cells and impairs oocyte competence via activation of the fas/fasl system
Chuan-Yong Li, Zhi-Bin Li, Qiao-Qiao Kong, Xiao Han, Bin Xiao, Xiao Li, Zhong-Le Chang, Jing-He Tan
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Abstract

Mechanisms by which psychological stress damages oocytes are largely undetermined. Although a previous study showed that the stress-induced corticotrophin-releasing hormone (CRH) elevation impaired oocyte competence by triggering apoptosis of ovarian cells, how CRH causes apoptosis in ovarian cells and oocytes is unknown. In this study, we have examined the hypothesis that restraint stress (RS)-induced CRH elevation triggers apoptosis of ovarian cells and impairs oocyte competence through activating the Fas/FasL system. The results showed that RS of female mice impaired oocyte competence, enhanced expression of CRH and CRH receptor (CRH-R) in the ovary, and induced apoptosis while activating the Fas/FasL system in mural granulosa cells (MGCs) and oocytes. Injecting mice with CRH-R1 antagonist antalarmin significantly alleviated the adverse effect of RS on oocyte developmental potential. Treatment of cultured MGCs recapitulated the effects of CRH and antalarmin on apoptosis and Fas/FasL expression in MGCs. Silencing FasL gene by RNA interference in cultured MGCs further confirmed the involvement of the Fas/FasL system in the CRH triggered apoptosis of ovarian cells. It is concluded that the RS-induced CRH elevation triggers apoptosis of ovarian cells and impairs oocyte competence via activation of the Fas/FasL system.

Summary Sentence

Both in vivo and in vitro trials demonstrated that the restraint stress-induced CRH elevation in female mice triggered apoptosis of ovarian cells and impaired oocyte competence via activation of the Fas/FasL system.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Chuan-Yong Li, Zhi-Bin Li, Qiao-Qiao Kong, Xiao Han, Bin Xiao, Xiao Li, Zhong-Le Chang, and Jing-He Tan "Restraint-induced corticotrophin-releasing hormone elevation triggers apoptosis of ovarian cells and impairs oocyte competence via activation of the fas/fasl system," Biology of Reproduction 99(4), 828-837, (16 April 2018). https://doi.org/10.1093/biolre/ioy091
Received: 9 February 2018; Accepted: 12 April 2018; Published: 16 April 2018
JOURNAL ARTICLE
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KEYWORDS
Apoptosis
CRH
Fas/FasL
oocyte competence
psychological stress
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