Translator Disclaimer
24 April 2018 Endocrine disruptor exposure during development increases incidence of uterine fibroids by altering DNA repair in myometrial stem cells
Lauren E Prusinski Fernung, Qiwei Yang, Daitoku Sakamuro, Alpana Kumari, Aymara Mas, Ayman Al-Hendy
Author Affiliations +
Abstract

Despite the major negative impact uterine fibroids (UFs) have on female reproductive health, little is known about early events that initiate development of these tumors. Somatic fibroid-causing mutations in mediator complex subunit 12 (MED12), the most frequent genetic alterations in UFs (up to 85% of tumors), are implicated in transforming normal myometrial stem cells (MSCs) into tumor-forming cells, though the underlying mechanism(s) leading to these mutations remains unknown. It is well accepted that defective DNA repair increases the risk of acquiring tumor-driving mutations, though defects in DNA repair have not been explored in UF tumorigenesis. In the Eker rat UF model, a germline mutation in the Tsc2 tumor suppressor gene predisposes to UFs, which arise due to “second hits” in the normal allele of this gene. Risk for developing these tumors is significantly increased by early-life exposure to endocrine-disrupting chemicals (EDCs), suggesting increased UF penetrance is modulated by early drivers for these tumors. We analyzed DNA repair capacity using analyses of related gene and protein expression and DNA repair function in MSCs from adult rats exposed during uterine development to the model EDC diethylstilbestrol. Adult MSCs isolated from developmentally exposed rats demonstrated decreased DNA end-joining ability, higher levels of DNA damage, and impaired ability to repair DNA double-strand breaks relative to MSCs from age-matched, vehicle-exposed rats. These data suggest that early-life developmental EDC exposure alters these MSCs' ability to repair and reverse DNA damage, providing a driver for acquisition of mutations that may promote the development of these tumors in adult life.

Summary Sentence

Effects of perinatal endocrine disruptor-exposure on DNA repair of adult rat myometrial stem cells(MSCs) were examined. MSCs from exposed animals showed increased DNA damage and diminished DNA repair.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Lauren E Prusinski Fernung, Qiwei Yang, Daitoku Sakamuro, Alpana Kumari, Aymara Mas, and Ayman Al-Hendy "Endocrine disruptor exposure during development increases incidence of uterine fibroids by altering DNA repair in myometrial stem cells," Biology of Reproduction 99(4), 735-748, (24 April 2018). https://doi.org/10.1093/biolre/ioy097
Received: 4 December 2017; Accepted: 20 April 2018; Published: 24 April 2018
JOURNAL ARTICLE
14 PAGES


Share
SHARE
KEYWORDS
CD44/Stro1
developmental reprogramming
DNA repair
endocrine disruptors
myometrial stem cells
uterine fibroids
RIGHTS & PERMISSIONS
Get copyright permission
Back to Top