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7 May 2018 Obesity in mares promotes uterine inflammation and alters embryo lipid fingerprints and homeostasis
Dawn R. Sessions-Bresnahan, Adam L. Heuberger, Elaine M. Carnevale
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Abstract

Maternal body composition can be an important determinant for development of obesity and metabolic syndrome in adult offspring. Obesity-related outcomes in offspring may include epigenetic alterations; however, mechanisms of fetal programming remain to be fully elucidated. This study was conducted to determine the impact of maternal obesity in the absence of a high fat diet on equine endometrium and preimplantation embryos. Embryos were collected from normal and obese mares at 8 and 16 days and a uterine biopsy at 16 days (0 day = ovulation). With the exception of 8 day embryos, each sample was divided into two pieces. One piece was analyzed for gene expression markers related to carbohydrate metabolism, lipid homeostasis, inflammation, endoplasmic reticulum stress, oxidative stress, mitochondrial stress, and components of the insulin-like growth factor (IGF) system. The second piece was analyzed for lipid content using matrix-assisted laser desorption/ionization mass spectrometry. Obese mares had elevated concentrations of insulin, leptin, and total cholesterol, and they tended to have increased triglycerides and decreased insulin sensitivity. Embryos from obese mares had altered transcript abundance in genes for inflammation and lipid homeostasis, as well as endoplasmic reticulum, oxidative and mitochondrial stress and altered lipid fingerprints. Endometrium from obese mares had increased expression of inflammatory cytokines, lipid homeostasis regulation, mitochondrial stress, and the IGF2 system. This study demonstrates that increased adiposity in mares alters the uterine environment, transcript abundance of genes for cellular functions, and lipid profiles of embryos. These alterations could affect prenatal programming, with potential long-term effects in offspring.

Summary Sentence

Excess adiposity is sufficient to increase transcript abundance of inflammatory cytokines in the uterine endometrium and alters embryonic lipid content, and gene expression for lipid metabolism, endoplasmic reticulum stress, and mitochondrial function.

© The Author(s) 2018. Published by Oxford University Press on behalf of Society for the Study of Reproduction. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
Dawn R. Sessions-Bresnahan, Adam L. Heuberger, and Elaine M. Carnevale "Obesity in mares promotes uterine inflammation and alters embryo lipid fingerprints and homeostasis," Biology of Reproduction 99(4), 761-772, (7 May 2018). https://doi.org/10.1093/biolre/ioy107
Received: 29 November 2017; Accepted: 4 May 2018; Published: 7 May 2018
JOURNAL ARTICLE
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KEYWORDS
ADIPONECTIN
embryo
equids
insulin
leptin
lipids
metabolomics
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