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1 September 2004 Research Article: The differential effects of nickel on 4 strains of the nematode, Caenorhabditis elegans.
JoAnne Brown
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Nickel concentration in the environment of Caenorhabditis elegans may affect its longevity. Furthermore, it would be expected that the genetic variants of C. elegans known to promote advanced longevity (clk-1, daf-2, and daf-2/clk-1) would be affected in an inverse proportion if the ability to detoxify nickel in the substrate were disrupted. Ten nematodes per variant were subjected to varying concentrations of nickel treated K Agar gel plates and monitored daily for 12 days for developmental disruptions and LC50. Four repetitions of this experiment were run simultaneously. Data from these tests were analyzed using TOXSTAT. The null hypothesis is that there is no significant difference between the sensitivity to nickel of the mutated C. elegans and the wild type. From the statistical analysis, the null hypothesis was rejected. Related research conducted by Barsyte showed an increase in heavy metal tolerance, specifically cadmium and copper, in 24-hour test trials with the wild type C. elegans. This research is in contrast to the results achieved using nickel in 12-day test trials, which could be due to the difference in length of the experiments and/or the heavy metals tested. Other nematode aging studies have shown that there is no tradeoff in fertility or reproductivity. There is an increase in life span that is related to starvation of the nematodes for the daf-2 mutation. Additional research in these areas may show a relationship between the mutations in these nematodes, nickel exposure, and the human disease genes that are known to be of similar genetic constitution.

JoAnne Brown "Research Article: The differential effects of nickel on 4 strains of the nematode, Caenorhabditis elegans.," BIOS 75(3), 95-102, (1 September 2004).<0095:TDEONO>2.0.CO;2
Received: 31 March 2003; Accepted: 1 November 2003; Published: 1 September 2004
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