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1 December 2004 Research Article: Beta-galactosidase staining as a marker of cells enduring stress
Michelle A. Wood, Jane F. Cavender
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The mechanism of senescence, permanent G1 arrest, is gradually becoming elucidated; however, the exact function of senescence remains unclear. A marker assay detecting the acidic senescence-associated beta-galactosidase (saβgal) has been used as an indicator for cellular senescence. However, more recently, others have shown that expression of this low pH saβgal may not be solely limited to senescent cells. This study has extended the findings on stress induction of saβgal by assessing the enzyme activity in additional species (monkey and rat) and determining if saβgal is stimulated by the stresses of temperature and alkalinity. Our results support previous data that saβgal is not solely a marker of senescence but may in fact be a highly conserved, ubiquitous marker of cellular stress. Additionally, we feel that our data, in conjunction with the literature, indicate that this sa (stress associated) βgal may play a pivotal role in multiple signal pathways in the cell.

Michelle A. Wood and Jane F. Cavender "Research Article: Beta-galactosidase staining as a marker of cells enduring stress," BIOS 75(4), 139-146, (1 December 2004).<0139:BSAAMO>2.0.CO;2
Received: 22 January 2004; Published: 1 December 2004
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