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1 September 2013 Overexpression of the kinetochore-associated proteins SKAP and astrin in human breast cancer
Christopher Wright, William S. Brooks
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The faithful alignment and segregation of chromosomes during mitosis is essential for the prevention of aberrant chromosome numbers that can lead to cancer. This process is mediated by the mitotic spindle, which forms stable attachments to the chromosomal centromere through the kinetochore structural intermediate. Small kinetochore-associated protein (SKAP) is a recently described component to the kinetochore that complexes with other kinetochore-associated proteins including astrin, CENP-E, and CEP55. SKAP is required for proper chromosome segregation and the metaphase to anaphase transition as its depletion results in activation of the spindle assembly checkpoint and mitotic delay. Here, we show that overexpression of SKAP in cultured cells speeds cell division in a colony formation assay. We also show that SKAP along with the interacting proteins CEP55 and astrin are overexpressed in human breast carcinoma. These data suggest that aberrant overexpression of SKAP and the astrin-SKAP complex may help facilitate the rapid growth of tumor cells.

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Christopher Wright and William S. Brooks "Overexpression of the kinetochore-associated proteins SKAP and astrin in human breast cancer," BIOS 84(3), 136-141, (1 September 2013).
Received: 26 July 2012; Accepted: 1 October 2012; Published: 1 September 2013

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