The faithful alignment and segregation of chromosomes during mitosis is essential for the prevention of aberrant chromosome numbers that can lead to cancer. This process is mediated by the mitotic spindle, which forms stable attachments to the chromosomal centromere through the kinetochore structural intermediate. Small kinetochore-associated protein (SKAP) is a recently described component to the kinetochore that complexes with other kinetochore-associated proteins including astrin, CENP-E, and CEP55. SKAP is required for proper chromosome segregation and the metaphase to anaphase transition as its depletion results in activation of the spindle assembly checkpoint and mitotic delay. Here, we show that overexpression of SKAP in cultured cells speeds cell division in a colony formation assay. We also show that SKAP along with the interacting proteins CEP55 and astrin are overexpressed in human breast carcinoma. These data suggest that aberrant overexpression of SKAP and the astrin-SKAP complex may help facilitate the rapid growth of tumor cells.
You have requested a machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Neither BioOne nor the owners and publishers of the content make, and they explicitly disclaim, any express or implied representations or warranties of any kind, including, without limitation, representations and warranties as to the functionality of the translation feature or the accuracy or completeness of the translations.
Translations are not retained in our system. Your use of this feature and the translations is subject to all use restrictions contained in the Terms and Conditions of Use of the BioOne website.
Vol. 84 • No. 3