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1 March 2014 The antimicrobial effect of anti-dnaK peptide nucleic acids on multidrug resistant strains of Escherichia coli and Salmonella enterica serovar Typhimurium
Dilara Kiran, Nammalwar Sriranganathan
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Abstract

Escherichia coli and Salmonella enterica species are Gram-negative bacteria that have recently shown acquired resistance to multiple antimicrobials. These resistant strains, referred to as multidrug resistant (MDR), are not effectively inhibited by current antimicrobials and therefore cause a range of untreatable infections. Peptide nucleic acids (PNA) have been proposed as an alternative antimicrobial treatment. These synthetic nucleic acid mimics are effective through steric inhibition of bacterial transcription and translation. This study examines the effect of PNA on MDR strains of E. coli and S. enterica serovar Typhimurium (S. Typhimurium). Kirby-Bauer disk diffusion susceptibility and minimum inhibitory concentration determinations (MIC) were completed to show bacterial resistance levels to current antimicrobials. Using S. Typhimurium specific dnaK PNA, MIC was determined to assess the effectiveness of PNA on MDR strains of E. coli and S. Typhimurium. Results showed that while the dnaK PNA was only mildly effective at inhibiting growth of MDR and control E. coli strains, there was a more noticeable effect of PNA on S. Typhimurium. Scrambled sequence control PNA did not have an effect. These results exemplify the species specificity of PNA and its ability to inhibit pathogenic bacteria in a targeted manner. Although future studies are still needed, the findings demonstrate that anti-dnaK PNA are an effective in vitro treatment for S. enterica strains, and that they are a possible alternative antimicrobial treatment for use against multidrug resistant bacterial infections.

Dilara Kiran and Nammalwar Sriranganathan "The antimicrobial effect of anti-dnaK peptide nucleic acids on multidrug resistant strains of Escherichia coli and Salmonella enterica serovar Typhimurium," BIOS 85(1), 48-56, (1 March 2014). https://doi.org/10.1893/0005-3155-85.1.48
Received: 21 September 2012; Accepted: 1 July 2013; Published: 1 March 2014
JOURNAL ARTICLE
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